| 货号 | AF7356-SP |
| 别名 | ACAM; ACAMCoxsackie- and adenovirus receptor-like membrane protein; Adipocyte adhesion molecule; adipocyte-specific adhesion molecule; ASAMCAR-like membrane protein; Asp5; CLMP; CXADR-like membrane protein; FLJ22415 | 全称 | Adipocyte-specific Adhesion Molecule |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human and mouse recombinant ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79827 (Human); 71566 (Mouse); 286939 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse ASAM Thr18-Ala234 Accession # Q8R373 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ASAM (Adipocyte-specific adhesion molecule; also ACAM, CLMP, Asp5 and CXADR-like protein) is a 44-48 kDa member of the CTX family of molecules. It is expressed on adipocytes as well as select epithelial cells such as respiratory and intestinal epithelium, keratinocytes and Sertoli cells. ASAM appears to contribute to tight junction formation, and may promote the transition of preadipocytes to adipocytes. Mature mouse ASAM is a 356 amino acid (aa) type I transmembrane glycoprotein. It contains a 217 aa extracellular region (aa 18-234) that is characterized by the presence of two C2 type Ig-like domains (aa 18-126 and 134-223), and possesses a C-terminal 118 aa cytoplasmic domain. The extracellular region of mouse ASAM (aa 18-234) shares 97% and 100% aa sequence identity with human and rat ASAM, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse ASAM by Western Blot. Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of ASAM in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). | |
Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). |
