货号 | AF7348-SP |
别名 | C2 calcium-dependent domain containing 3 | 全称 | C2 Domain-containing Protein 3 |
反应种属 | Human |
应用 | Immunocytochemistry(5-15 µg/mL) |
目标/特异性 | Detects human C2CD3 in direct ELISAs. |
使用方法 | Immunocytochemistry: 5-15 µg/mL |
来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 26005 (Human); 277939 (Mouse); 293148 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human C2CD3 Met1-Asp109 Accession # Q4AC94 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
背景 | C2CD3 (C2 domain-containing protein 3) is a 260 kDa (predicted) intracellular protein that apparently belongs to no known structural protein family. It is ubiquitously expressed, and plays a role in cilia formation and associated hedgehog signaling. In particular, C2CD3 is localized to the basal body, and appears to promote Gli3 proteolytic processing into a truncated transcriptional repressor. Human C2CD3 is 2353 amino acids (aa) in length. It contains two C2 domains (aa 1163-1289 and 1622-1728) that participate in Ca++-dependent protein-lipid and protein-protein interactions. Three utilized phosphorylation sites exist at Thr1130, Ser2114 and Ser 2132. There are multiple potential splice variants. One possesses a three aa substitution for aa 1961-2353, a second shows a deletion of aa 1364-1382 coupled to a 27 aa substitution for aa 1881-2353, a third contains a seven aa substitution for aa 1961-2353, and a fourth contains a 34 aa substitution for aa 1888-2353. Over aa 1-109, human C2CD3 shares 94% aa identity with mouse C2CD3. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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C2CD3 in HeLa Human Cell Line. C2CD3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human C2CD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |