| 货号 | AF7317-SP |
| 别名 | FLJ22774; MGC119595; MGC119596; MGC119597,4832410J21Rik; SLIT and NTRK-like family, member 6; SLIT and NTRK-like protein 6; slit and trk like gene 6 | 全称 | SLIT and NTRK-like Family, Member 6 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human SLITRK6 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SLITRK2, rhSLITRK4, and rhSLITRK6 is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84189 (Human); 239250 (Mouse); 290467 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human SLITRK6 Ile16-Ala604 Accession # Q9H5Y7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SLITRK6 (Slit and NTRK-like 6) is a 95 kDa (predicted) member of the SLITRK family of proteins. It is widely expressed in the embryo, including cells in limb bud mesenchyme, visceral organ primodia, cells of the dorsal thalamus, and cochlear plus vestibular sensory epithelium. In the ear, SLITRK6 is suggested to induce neurotrophin expression from cells, thus promoting sensory neuron survival and axon pathfinding. Mature human SLITRK6 is an 826 amino acid (aa) type I transmembrane protein. It contains a 593 aa extracellular domain (ECD) (aa 16-608) plus a 212 aa cytoplasmic region. The ECD possesses two LRR clusters, one that involves seven LRRs (aa 27-269), and a second that involves eight LRRs (aa 320-568). Over aa 16-604, human SLITRK6 shares 89% aa sequence identity with mouse SLITRK6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SLITRK6 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human SLITRK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLITRK6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
