| 货号 | AF73091-SP |
| 别名 | HELIOS; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; subfamily 1A, 2 (Helios); zinc finger DNA binding protein Helios; zinc finger protein Helios; ZNF1A2; ZNFN1A2 | 全称 | DNA-binding Protein Helios |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(4 µg/mL) |
| 目标/特异性 | Detects human Helios in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 4 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22807 (Human); 22779 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Helios Ile6-Gln97 Accession # Q9UKS7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Helios, also known as IKZF2, is a 70 kDa DNA-binding transcription regulator in the Ikaros family that contains four N-terminal C2H2-type zinc finger domains (amino acids 112-219) and two C-terminal zinc finger domains (amino acids 471-523). Helios is expressed in developing hematopoietic and epithelial tissues and in adult T cells and thymic-derived regulatory T cells (Treg). It forms homodimers and also heterodimers with other Ikaros family proteins Ikaros, Pegasus, Eos, and Aiolos. Alternate splicing of human Helios generates a short isoform that lacks three of the the N-terminal zinc finger domains. This isoform is overexpressed in T cell leukemias where it can still dimerize with Ikaros proteins but functions as a dominant negative regulator. Within amino acids 6-97, human and mouse Helios share 96% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat lysates, MOLT‑4 lysates, or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation. | |
Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat or MOLT‑4 lysates were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation. |
