| 货号 | AF7296-SP |
| 别名 | DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein | 全称 | Guanylate Kinase-associated Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human GKAP/DLGAP1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DLGAP2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9229 (Human); 224997 (Mouse); 65040 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GKAP/DLGAP1 Lys368-Glu472 Accession # O14490 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DLGAP1 (Disks Large-associated protein 1; also SAPAP1, GKAP and SAP90 binding protein) is an intracellular, 117-130 kDa member of the SAPAP family of proteins. It is widely expressed (astrocytes, neurons, fibroblasts and epithelium), and serves as a platform for dynein-DGL1 interaction. This is important during cell migration where the centrosome must be positioned between the nucleus and direction of migration in response to microtubule formation under the protruding edge of the cell membrane. DLGAP1 first binds DLG-1 and then recruits dynein, with a resultant interaction that allows for microtubule anchoring and centrosome positioning. Human DLGAP1 is 977 amino acids (aa) in length. It contains multiple 14 aa acidic repeats, three Pro-rich segments, and at least 14 potential phosphorylation sites. Multiple potential splice variants are reported. In various combinations, there may be a 17, 27 and 31 aa substitution for aa 1-319, a deletion of either aa 531-540 or 531-558, a 24 aa substitution for aa 1-318, and a 13 aa substitution for aa 531-977. Most appear to generate 70-80 kDa isoforms in SDS-PAGE. Over aa 368-472, human and mouse DLGAP1 are identical in aa sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GKAP/DLGAP1 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GKAP/DLGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7296) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific band were detected for GKAP/DLGAP1 at approximately 80 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
