| 货号 | AF7285-SP |
| 别名 | D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; EC 3.1.3; EC 3.1.3.11; FBP; FBP1; FBPase 1; fructose-1,6-bisphosphatase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17 | 全称 | Fructose-1,6-bisphosphatase 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human, mouse, rat FBPase 1 in Western blots and detects human FBPase 1 in direct ELISAs. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2203 (Human); 14121 (Mouse); 24362 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FBPase 1 Ala2-Gln338 (Arg218Lys) Accession # P09467 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | FBPase 1 (Fructose 1,6 bisphosphatase 1 [Fru-1,6-P2ase]; also FBP-1 phosphohydrolase 1) is a 35-37 kDa member of the FBPase Class I family of enzymes. It is widely expressed, being found in cells diverse as monocytes, hepatocytes, pancreatic beta -cells and striated (skeletal plus cardiac) muscle cells. FBPase 1 converts Fru-1,6-P2 into Fru-6-P, a molecule that is subsequently used to generate glucose. Human FBPase 1 is 338 amino acids (aa) in length. It contains one catalytic region (aa 18-332) and an NLS between aa 203-208. FBPase 1 exhibits divalent cation dependency, and exists as a homotetramer within the cell. Full-length human FBPase 1 shares 85% aa sequence identity with mouse FBPase 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat FBPase 1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat FBPase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FBPase 1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
