| 货号 | AF7280-SP |
| 别名 | calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CAMK; CAMK2G; CAMKG; CAMKGFLJ16043; CaMK-II subunit gamma; CAMK-II; EC 2.7.11; EC 2.7.11.17; MGC26678 | 全称 | Calcium/calmodulin-dependent Protein Kinase II gamma Subunit |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CaM Kinase II gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) CaM Kinase II beta is observed, and less than 5% cross-reactivity with rhCaM Kinase II alpha and rhCaM Kinase II δ is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 818 (Human); 12325 (Mouse); 171140 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CaM Kinase II gamma Ala448-Gln558 Accession # Q13555 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Calcium/calmodulin-dependent protein kinase type II gamma (CaMKII gamma ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. The widely expressed CaMKII gamma from human, mouse, and rat share 100% aa sequence identity within aa 448-558 of isoform 1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CaM Kinase II gamma by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase II gamma at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
