| 货号 | AF7278-SP |
| 别名 | Cadherin family member 11; cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila); CDHF11; CDHF11RESDA1; EGFL1; EGFL1cadherin EGF LAG seven-pass G-type receptor 3; EGF-like protein 1; EGF-like-domain, multiple 1; epidermal growth factor-like 1; Epidermal growth factor-like protein 1; Flamingo homolog 1; FMI1anchor protein; HFMI1; KIAA0812; MEGF2; MEGF2cadherin EGF LAG seven-pass G-type receptor 3, flamingo (Drosophila) homolog; multiple EGF-like domains 2; Multiple EGF-like domains protein 2; Multiple epidermal growth factor-like domains protein 2; RESDA1 | 全称 | Cadherin, EGF LAG Seven-pass G-type Receptor 3 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human CELSR3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR2 is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1951 (Human); 107934 (Mouse); 83466 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CELSR3 Tyr531-Ser711 Accession # Q9NYQ7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CELSR3 (Cadherin EGF LAG Seven-pass G-type Receptor 3; also cadherin family member 11/CDHF11, FMI1 and EGFL1) is a 355 kDa (predicted) member of the LN-7TM subfamily, GPCR 2 family of proteins. It is coexpressed with Fzd3 on postmigratory neurons of the developing DRG and cranial ganglia, and appears to serve as an axonal guidance cue. Mature human CELSR3 is 3280 amino acids (aa) in length (aa 33-3312). It is a highly complex 7-transmembrane protein that contains a 2508 aa extended N-terminal extracellular region (aa 33-2540) plus a 538 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 326-1265), followed by a mixture of eight EGF-like and three laminin-like domains, and one membrane-proximal GPS domain. There are two potential isoform variants. One shows a five aa insertion after Gly2158, while another possesses an alternative start site at Met276. Over aa 531-711, human CELSR3 shares 97% aa sequence identity with mouse CELSR3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of CELSR3 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7278, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
