Human TNF RII/TNFRSF1B Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TNF RII/TNFRSF1B Affinity Purified Polyclonal Ab (25 UG)

货号： AF726-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF726-SP
别名	CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
全称	Tumor Necrosis Factor Receptor II
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human TNF RII/TNFRSF1B in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse TNF RII is observed and less than 5% cross-reactivity with recombinant rat TNF RII is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 µg/mL in the presence of 0.075 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation. Authors: Horton J, Hudak K, Chung E, White A, Scroggins B, Burkeen J, Citrin D Stem Cells, 2013;31(10):2231-41. Species: Mouse Sample Type: Whole Cells Application: Blocking
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TNF RII/TNFRSF1B
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TNF RII, also known as p75 or p80 TNF R, is a prototypic member of theTNF receptor superfamily and has been designated TNFRSF1B. It binds TNFwith high affinity and is expressed primarily in cells of the immunesystem.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell Structure and Signaling Molecules Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RII/TNFRSF1B Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) T Cell Cytokine Signaling TNF Superfamily TNF Superfamily Receptors

参考图片

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog #
726‑R2) inhibits Recombinant Human TNF‑ alpha (Catalog #
210‑TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.075 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726). The ND₅₀ is typically
0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).