| 货号 | AF7257-SP |
| 别名 | EC 3.5.3.15; KIAA0994PDI2PAD2; PAD2; PAD-H19; PDI2; peptidlyarginine deiminase type II; peptidyl arginine deiminase, type II; Peptidylarginine Deiminase II; protein arginine deiminase; Protein-arginine deiminase type II; protein-arginine deiminase type-2 | 全称 | Peptidyl Arginine Deiminase type II |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PADI2 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11240 (Human); 18600 (Mouse); 29511 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PADI2 Met1-Ala115 Accession # Q9Y2J8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PADI2 (Protein-arginine deiminase type 2; also PAD2, PDI2 and PAD-H19) is a 72-76 kDa member of the PAD family of proteins. It is widely expressed, being found in macrophages, astrocytes, select neurons, skeletal muscle and bronchial epithelial cells, and keratinocytes. PADI2 catalyzes the Ca++-dependent conversion of guanidino groups (NH2-C[=NH]-NH−) to ureido groups (NH2-C[=O]-NH-) on peptidylArg, a posttranslational process known as citrullination. Human PADI2 is 665 amino acids (aa) in length. It contains an N-terminal PAD domain (aa 1-114), a middle PAD-M domain (aa 116-274), and a catalytic region (aa 279-665). Over aa 1-115, human PADI2 shares 90% aa sequence identity with mouse PADI2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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PADI2 in Human Brain. PADI2 was detected in immersion fixed paraffin-embedded sections of human Alzheimers brain (cortex) using Sheep Anti-Human PADI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7257) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
