| 货号 | AF7256-SP |
| 别名 | diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2 (mouse); diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; Diglyceride acyltransferase 2; DKFZp686A15125; EC 2.3.1; EC 2.3.1.20; GS1999FULL | 全称 | Diacylglycerol O-Acyltransferase 2 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DGAT2 in direct ELISAs. In direct ELISAs, approximately 7% cross-reactivity with recombinant human DGAT2-L6 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84649 (Human); 67800 (Mouse); 252900 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DGAT2 Arg268-Phe377 Accession # Q96PD7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DGAT2 (Diacylglycerol O-acyltransferase 2; also DGAT2 alpha ) is a 45-50 kDa, insulin-regulated member of the DGAT2 family of enzymes. Although DGAT1 also exists, it is related to DGAT2 only by function; DGAT1 and DGAT2 are members of structurally unrelated gene families. DGAT2 catalyzes the formation of triglyceride (TG) from sn-1, 2-DG and a variety of acyl-CoAs. It is found in sebaceous gland cells, adipocytes, and hepatocytes, and appears to synthesize TGs for incorporation into VLDL particles. Human DGAT2 is a 388 amino acid (aa) multipass ER protein. It contains a 69 aa N-terminal cytoplasmic region, a short luminal sequence (aa 89-92) and a long C-terminal cytoplasmic domain that contains acyl transferase activity (aa 113-388). DGAT2 does homomultimerize, forming 680 kDa complexes. There is one splice variant that contains a deletion of aa 41-83. Over aa 268-377, human DGAT2 shares 95% aa sequence identity with mouse DGAT2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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DGAT2 in HepG2 Human Cell Line. DGAT2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
DGAT2 in Human Liver. DGAT2 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
