| 货号 | AF7227-SP |
| 别名 | CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human CD34 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD34 and recombinant rat CD34 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human CD34 Ser32-Thr290 Accession # P28906 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CD34 is a 105-115 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, stem cells and renal mesangial cells. Mature human CD34 is a 354 amino acid (aa) type I transmembrane protein (aa 32-385). It contains a 259 aa extracellular region (aa 35-287) with utilized N- and O-linked glycosylation sites, and a 74 aa cytoplasmic domain that may undergo Tyr phosphorylation. There is one splice variant that shows a four aa substitution for aa 325-385. Human CD34 can undergo membrane cleavage by bacterial proteases to generate 30-40 kDa soluble fragments. And notably, desialylated CD34 shows a 40 kDa increase in MW (to 150 kDa) when run in SDS-PAGE. Over aa 32-290, human CD34 shares 56% aa identity with mouse CD34. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CD34 in Human Liver. CD34 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7227) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
