| 货号 | AF7216-SP |
| 别名 | enhancer of split groucho 2; ESG; ESG2; ESG2Enhancer of split groucho-like protein 2; FLJ41188; GRG2; transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 2, homolog of Drosophila E(sp1); transducin-like enhancer protein 2 | 全称 | Transducin-like Enhancer of Split 2 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TLE2 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TLE1, rhTLE3, and rhTLE4 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7089 (Human); 21886 (Mouse); 299636 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TLE2 Ile11-Ser193 Accession # Q04725 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TLE2 (Transducin-Like Enhancer of Split 2; also ESG2 and Grg-2) is an 83-84 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is ubiquitously expressed, and is known to antagonize TCF (Wnt)-mediated signaling. TLE2 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of multiple factors such as FoxG1, Arx and histone H3. Human TLE2 is 743 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa 1-133), a CCN domain that contains an NLS (aa 195-256), and six WD repeats that mediate protein-protein interaction (aa 455-742). There are at least five potential phosphorylation sites that if used, may account for SDS-Page MWs exceeding 95 kDa. Potential isoform variants exist. One possesses a 21 aa substitution for aa 1-9, while a second shows the same substitution coupled to an additional 10 aa substitution for aa 683-743. A third isoform possesses an alternative start site at Met56 coupled to a deletion of aa 124-190. Over aa 11-193, human TLE2 shares 91% aa identity with mouse TLE2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TLE2 in Human Lung Mesothelioma. TLE2 was detected in immersion fixed paraffin-embedded sections of human lung mesothelioma using Sheep Anti-Human TLE2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7216) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
