Human ICAM-1/CD54 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human ICAM-1/CD54 Affinity Purified Polyclonal Ab (25 UG)

货号： AF720-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF720-SP
别名	BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
全称	Intercellular Adhesion Molecule 1
反应种属	Human
应用	Western Blot(0.2 µg/mL)
目标/特异性	Selected for its ability to block the adhesion of HSB‑2 cells to immobilized recombinant human (rh) ICAM‑1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM‑1, recombinant mouse ICAM‑1, and rhVCAM-1 is observed.
使用方法	Western Blot: 0.2 µg/mL Adhesion Blockade: The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 10⁴ cells/well) to immobilized recombinant human ICAM-1/Fc Chimera (12.5 μg/mL, 100 µL/well) was maximally inhibited (80‑100%) by 25 μg/mL of the antibody.
来源	Polyclonal Sheep IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1. Authors: Hashizume M, Mihara M Biochem. Biophys. Res. Commun., 2010;403(2):184-9. Species: Human Sample Type: Whole Cells Application: Neut C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F J. Leukoc. Biol., 2008;84(4):1109-19. Species: Human Sample Type: Whole Cells Application: Neut The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium. Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE Arthritis Rheum., 2007;56(6):1817-26. Species: Human Sample Type: Cell Lysates Application: WB Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm. Authors: Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R Blood, 2007;109(4):1381-6. Species: Human Sample Type: Whole Cells Application: Neut Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E Lab. Invest., 2007;87(11):1159-70. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1 Extracellular domain
内毒素水平	<0.20 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adhesion Molecules Adhesive Interactions in Allergy and Asthma Adipose-derived Stem Cell Markers B Cell Chemotaxis, Migration, and Adhesion Dendritic Cell Adhesion and Migration Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Human CD Marker Products ICC / IHC Images: ICAM-1/CD54 Immunoglobulin Superfamily CAMs Mesenchymal Stem Cell Markers Mini-review: Cytolytic Granule-mediated Apoptosis Monocyte Markers Myeloid-derived Suppressor Cells (MDSC) Neuroinflammation Plasma ICAM-1 is Correlated with Increased Risk of Cardiovascular Disease Platelets Regulatory T Cells (Tregs) T Cell Migration/Adhesion

参考图片

Detection of Human ICAM‑1/CD54 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitts lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA) for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.