| 货号 | AF7209-SP |
| 别名 | DKFZp566O011; EC 2.3.2.2; gamma-glutamyl cleaving enzyme; Gamma-glutamyl transpeptidase-related enzyme; gamma-glutamyl transpeptidase-related protein; gamma-glutamyltransferase 5; Gamma-glutamyltransferase-like activity 1FLJ92733; Gamma-glutamyltranspeptidase 5; GGT 5; GGTLA1; GGT-rel | 全称 | gamma-Glutamyltransferase 5 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human GGT5 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2687 (Human); 23887 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GGT5 Ser30-Tyr586 Accession # P36269 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | GGT5 (Gamma GlutamylTranspeptidase 5; also GGL, GGT-rel and gamma -leukotrienease) is a 74-77 kDa member of the gamma -glutamyltransferase family of enzymes. It is suggested that multiple cell types express GGT5, including neurons, lymphocytes, myocardium and bronchial epithelium. It is also expressed on the surface of endothelial cells, and serves to both metabolize GSH to CysGly, and convert LTC4 to LTD4, an action that appears necessary for targeted inflammatory cell migration. Human GGT5 is a 586 amino acid (aa) type II transmembrane (TM) glycoprotein. It undergoes processing to generate a disulfide-linked heterodimer composed of a 56-58 kDa membrane-embedded heavy chain (aa 1-387, with the TM segment lying between aa 9-29) and a 20-21 kDa light chain (aa 388-586). There is one potential splice variant that shows a deletion of aa 112-133. Over aa 30-586, human GGT5 shares 78% aa identity with mouse GGT5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GGT5 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GGT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GGT5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
