| 货号 | AF7206-SP |
| 别名 | EMCN; EMCN2; endomucin; MUC-14 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Endomucin is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51705 (Human); 59308 (Mouse); 295490 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Endomucin Asn19-Gly139 Accession # Q9ULC0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Endomucin (Endothelial sialomucin; also MUC-14, Endomucin-2 and Ga34) is an 85-115 kDa glycoprotein that belongs to no known family of molecules. It is expressed by primitive bone marrow stem cells, endothelial cells of both blood and lymphatic vessels, and select keratinocytes. Endomucin appears to create an environment that discourages leukocyte binding to vascular walls, and yet may also serve as an L-Selectin ligand. Mature human Endomucin is a 243 amino acid (aa) type I transmembrane glycoprotein (aa 19-261). It contains a 172 aa extracellular domain (ECD) (aa 19-190) plus a 50 aa cytoplasmic tail (aa 212-261). The ECD possesses a uteroglobin-like domain (aa 19-74) plus multiple sites for N- and O-linked glycosylation. There are potential splice variants that seem to parallel those in mouse. One shows a deletion of aa 140-222 and is likely soluble, while another possesses a Ser substitution for aa 126-139. Over aa 19-139, human Endomucin shares only 35% aa identity with mouse Endomucin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Endomucin by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
