Human CCL28 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CCL28 Affinity Purified Polyclonal Ab (25 UG)

货号： AF717-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF717-SP
别名	CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CCL28 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 40-100 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56477 (Human); 56838 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa. Authors: Danilova, E, Skrindo, I, Gran, E, Hales, B J, Smith, W A, Jahnsen, J, Johansen, F E, Jahnsen, F L, Baekkevold, E S Mucosal Immunol, 2015;8(1):107-14. Species: Human Sample Type: tissue Application: IHC CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O J. Immunol., 2003;170(3):1452-61. Species: Human Sample Type: Milk Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL28 Ile23-Tyr127 Accession # Q9NRJ3
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wang, W.et al. (2000) J. Biol. Chem. 275:22313.

参考图片

Chemotaxis Induced by CCL28 and Neutralization by Human CCL28 Antibody. Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL28 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717). The ND₅₀ is typically 40-100 µg/mL.