| 货号 | AF7179-SP |
| 别名 | CD242 antigen; CD242; ICAM-4; intercellular adhesion molecule 4 (Landsteiner-Wiener blood group); intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4; intercellular adhesion molecule 4, Landsteiner-Wiener blood group; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; Landsteiner-Wiener blood group; LWLW blood group protein | 全称 | Intercellular Adhesion Molecule 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(10 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human ICAM-4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 10 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3386 (Human); 78369 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4 Ala31-Ala240 Accession # Q14773 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ICAM-4 (intercellular adhesion molecule 4; also Landsteiner-Wiener glycoprotein and CD242) is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ICAM‑4 by Western Blot. Western blot shows lysates of human erythrocytes and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM‑4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry. Human red blood cells (erythrocytes) were stained with Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). | |
Detection of Human ICAM‑4 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for ICAM‑4 at approximately 48 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
