| 货号 | AF7164-SP |
| 别名 | Angiotensinase C; EC 3.4.16.2; HUMPCP; Lysosomal carboxypeptidase C; lysosomal Pro-X carboxypeptidase; PCPMGC2202; Proline carboxypeptidase; prolylcarboxypeptidase (angiotensinase C); prolylcarboxypeptidase isoform 1 preproprotein; Prolylcarboxypeptidase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Lysosomal Pro‑X Carboxypeptidase/PRCP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5547 (Human); 72461 (Mouse); 293118 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Lysosomal Pro‑X Carboxypeptidase/PRCP Met1-His496 Accession # P42785 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PRCP (Prolylcarboxypeptidase; also PCP, lysosomal Pro-X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57-62 kDa glycoprotein member of the S28 family of proteases. It is expressed by a number of cells, including fibroblasts, macrophages, and endothelial cells. PRCP cleaves a variety of single C‑terminal amino acids (aa) adjacent to a Pro residue, and is known to act on, and inactivate, peptides such as prekallikrein, alpha -MSH and angiotensin II plus III. Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa 1-21), a short prosequence (aa 22-45) and a 451 aa mature region (aa 46-496). The mature region demonstrates a Ser-carboxypeptidase domain (aa 55-477) that, in itself, possesses an SKS domain that caps the enzymes catalytic site. The molecule is known to be mannosylated, and to form noncovalent homodimers. There is one splice variant that contains a 21 aa insertion after Lys56. Over aa 1-496, human and mouse PRCP share 77% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Lysosomal Pro‑X Carboxypeptidase/PRCP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Lysosomal Pro‑X Carboxypeptidase/PRCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysosomal Pro‑X Carboxypeptidase/PRCP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
