| 货号 | AF7116-SP |
| 别名 | EC 2.4.2.30; PARP5A; PARP-5a; PARP5APARPL; PARPL; pART5; Poly [ADP-ribose] polymerase 5A; TANK1; Tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; tankyrase-1; TIN1TANK1; TINF1; TINF1TNKS-1; TNKS; TNKS1TRF1-interacting ankyrin-related ADP-ribose polymerase | 全称 | TRF1-interacting Ankyrin-related ADP-ribose Polymerase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Tankyrase 1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8658 (Human); 21951 (Mouse); 290794 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Tankyrase 1 Ala943-Gly1039 Accession # O95271 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | TNKS (Tankyrase; also TRF1-interacting, ANKYrin-Related ADP-ribose polymerase and TNKS1, TANK1 and PARP5A) is a 165-170 kDa member of the PARP family of proteins. It is ubiquitously expressed and participates in at least two critical cellular functions. First, via ribosylation activity, it inhibits TRF1, a regulator of telomere length. This promotes chromosomal lengthening. Second, it activates the Wnt signaling pathway by promoting Axin-1 and -2 degradation, resulting in beta -catenin activation. Human TNKS is 1327 amino acids (aa) in length. It contains polyHis, Pro and Ser regions (aa 9-134), 15 ankyrin repeats (aa 215-934), one SAM motif (aa 1030-1089) and a PARP catalytic domain (aa 1112-1317). There are three potential splice variants. One shows a three aa substitution for aa 640-1327, another contains a 43 aa substitution for aa 1-327, while a third possesses a 20 aa substitution for aa 300-327. Over aa 943-1039, human TNKS shares 97% aa identity with mouse TNKS. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Tankyrase 1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tankyrase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tankyrase 1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
