| 货号 | AF7070-SP |
| 别名 | 180 kDa protein downstream of CRK; ced5; dedicator of cytokinesis 1; dedicator of cyto-kinesis 1; DOCK180; DOCK180dedicator of cytokinesis protein 1; DOwnstream of CrK | 全称 | Dedicator of Cytokinesis Protein 1 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects recombinant human DOCK1 in direct ELISAs. Detects human and rat DOCK1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1793 (Human); 330662 (Mouse); 309081 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DOCK1 Gly422-Ser666 Accession # Q14185 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DOCK1 (Dedicator of cytokinesis protein 1) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Although its predicted molecular weight is 215 kDa, it runs anomalously at 180-190 kDa in SDS-PAGE. It is generally found in non-hematopoietic cell types, and transduces signals from cell membrane receptors by regulating the activity of the GTPases Rac-1 and -2. This impacts both cell migration and phagocytosis. Human DOCK1 is 1865 amino acids (aa) in length. It contains one protein-interaction SH3 domain (aa 9-70), a DHR-1 region that localizes DOCK1 to the plasma membrane and PI3 kinase (aa 422-664), a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa 1111-1616), and two SH3-binding motifs (aa 1799-1843). There are four potential phosphorylation sites and one isoform variant that shows a start site at Met114. Over aa 422-666, human DOCK1 shares 97% aa identity with mouse DOCK1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat DOCK1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, C6 rat glioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DOCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DOCK1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
