| 货号 | AF7069-SP |
| 别名 | DAG kinase epsilon; DAGK5; DAGK6; DGK; DGKE; DGK-epsilon; diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon (64kD); diacylglycerol kinase, epsilon 64kDa; Diglyceride kinase epsilon; EC 2.7.1.107 | 全称 | Diacylglycerol Kinase, epsilon 64kDa |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human DGK-epsilon in direct ELISAs. Detects human and mouse DGK-epsilon in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8526 (Human); 56077 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DGK-epsilon Asn314-Arg435 Accession # P52429 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DGK-epsilon (Diacylglycerol kinase epsilon) is a 65 kDa member of the eukaryocytic diacylglycerol kinase family of enzymes. It is a type III DGK that possesses only a C1/Cys-rich domain and a catalytic region, and is found in neurons and testis. DGK-epsilon specifically phosphorylates arachidonate-containing DAG, and may downregulate DAG signaling that results from inositol cycling. Human DGK-epsilon is 567 amino acids (aa) in length. It would appear to contain one transmembrane domain (aa 22-42), two C1 DAG-binding regions (aa 59-108 and 124-177) and one catalytic domain (aa 219-350). DGK-epsilon apparently can form intramembrane oligomers. Over aa 314-435, human DGK-epsilon shows 99% aa identity to mouse DGK-epsilon. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse DGK‑ epsilon by Western Blot. Western blot shows lysates of Caki‑2 human clear cell carcinoma epithelial cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DGK‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DGK‑ epsilon at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
DGK‑ epsilon in Human Brainstem. DGK‑ epsilon was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human DGK‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7069) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
