| 货号 | AF7068-SP |
| 别名 | aristaless related homeobox; Aristaless-related homeobox; aristaless-related homeobox, X-linked; cancer/testis antigen 121; CT121; EIEE1; homeobox protein ARX; ISSX; ISSXmental retardation, X-linked 36; mental retardation, X-linked 29; mental retardation, X-linked 32; mental retardation, X-linked 33; mental retardation, X-linked 38; mental retardation, X-linked 43; mental retardation, X-linked 54; mental retardation, X-linked 76; mental retardation, X-linked 87; MRX29; MRX32; MRX33; MRX36; MRX38; MRX43; MRX54; MRX76; MRX87; MRXS1; PRTS | 全称 | Aristless-related Homeobox Protein |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ARX in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 170302 (Human); 11878 (Mouse); 317268 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ARX Ser2-Ala100 Accession # Q96QS3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ARX (Aristaless-Related homeoboX protein) is a 56-62 kDa member of the aristaless subfamily, paired homeobox family of proteins. In the nervous system, it is expressed in embryonic telencephalon plus adult cortical GABAergic neurons and differentiating neuronal stem cells; in the pancreas, it promotes the differentiation of alpha -cells at the expense of beta -cells. Human ARX is a 562 amino acid (aa) transcriptional repressor. It contains an octapeptide motif that binds Groucho proteins (aa 27‑34), an NLS (aa 82-89), one Ala-rich region (aa 100-155), an acidic/Glu-rich region (aa 224-253), a DNA-binding homeodomain with an embedded NLS (aa 328‑387), one Pro-rich segment that binds CtBP1 (aa 395-459) and a C-terminal Aristaless domain (aa 530-543). There are mutations that show poly-Ala expansions in the N-terminus (aa 115-155), often associated with developmental abnormalities. Over aa 1-100, human ARX shares 97% aa identity with mouse ARX. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ARX by Western Blot. Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARX at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ARX in U‑87 MG Human Cell Line. ARX was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
ARX in Human Pancreas. ARX was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human ARX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7068) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
