Human FUCA1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human FUCA1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF7039-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF7039-SP
别名	Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; tissue alpha-L-fucosidase
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Tissue alpha -L-Fucosidase/FUCA1 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2517 (Human); 71665 (Mouse); 24375 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Tissue alpha -L-Fucosidase/FUCA1 Gln32-Lys466 Accession # P04066
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hiraishi, K. et al. (1993) Glycobiology 3:381. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106. O’Brien, J.S. et al. (1987) Enzyme 38:45. Durand, P. et al. (1969) J. Pediatr. 75:665. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90. Shah, M. et al. (2008) Cancer 113:336.

参考图片

Detection of Human Tissue alpha ‑L‑Fucosidase/FUCA1 by Western Blot. Western blot shows lysates of human thyroid tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tissue alpha ‑L‑Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tissue alpha ‑L‑Fucosidase/FUCA1 in HeLa Human Cell Line. Tissue alpha ‑L‑Fucosidase/FUCA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.