| 货号 | AF7037-SP |
| 别名 | KIAA0524SAM domain-containing protein 2; SAMD2; SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila; SARMFLJ36296; Sterile alpha and Armadillo repeat protein; sterile alpha and TIR motif containing 1; sterile alpha and TIR motif-containing protein 1; Sterile alpha motif domain-containing protein 2; Tir-1 homolog | 全称 | Sterile alpha and TIR Motif Containing 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(4 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SARM1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 4 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23098 (Human); 237868 (Mouse); 287545 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SARM1 Lys556-Thr724 Accession # Q6SZW1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SARM1 (Sterile alpha and TIR motif-containing protein 1; also SAM domain-containing protein 2 and TIR-1 homolog) is a 73-75 kDa member of the TLR adaptor family of molecules. It is both nuclear and cytoplasmic, and expressed in monocytes, neurons, and retinal photoreceptor cells, where it demonstrates disparate activities. In monocytes, SARM1 is a specific inhibitor of TRIF-dependent TLR-3 and -4 signaling, and appears to block MAPK phosphorylation. In neurons, SARM1 regulates microtubule stability, and thus axon and dendrite elongation. And in retinal photoreceptor cells, SARM1 complexes with Na/K ATPase to create a cell surface receptor for retinoschisin. Human SARM1 is 724 amino acids (aa) in length. It contains an N-terminal polybasic motif (aa 1-20) and a Gly-rich region (aa 22-90) that may anchor the molecule to intracellular membranes. It also possesses two SAM domains (aa 412-548) plus a TIR region (aa 559-657) that appears to interact with TIRF. Proteolytic cleavage products of 35 kDa and 30 kDa have been described. There is one splice variant that shows a 72 aa substitution for aa 1-106. Over aa 556-724, human SARM1 shares 94% aa identity with mouse SARM1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Immunoprecipitation of Human SARM1. SARM1 was immunoprecipitated from 400 µg of SH‑SY5Y human neuroblastoma cell line lysates using 4 µg of Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). SH‑SY5Y lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated SARM1 was detected by Western blot under reducing conditions using 1.0 µg/mL Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation. | |
SARM1 in HEK293 Human Cell Line. SARM1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
