| 货号 | AF7034-SP |
| 别名 | CDA019; cystine-knot containing secreted protein; DKFZp564D206; Ectodermal BMP inhibitor; Ectodin; sclerostin domain containing 1; sclerostin domain-containing protein 1; SOSTDC1; USAG-1; USAG1uterine sensitization-associated protein-1; Uterine sensitization-associated gene 1 protein; WISE | 全称 | Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human USAG1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 25928 (Human); 66042 (Mouse); 266803 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human USAG1 Phe24-Ser206 Accession # Q6X4U4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | USAG1 (also Ectodin and WISE; Wnt modulator in surface ectoderm) is a secreted, monomeric 26-32 kDa member of the sclerostin family of proteins. It exhibits restricted expression, being found in renal distal tubule cells (including macula densa), ameloblasts of teeth (for enamel formation), and cells of the dermal papilla. USAG1 binds BMP-2,-4,-6,and -7 with high affinity. This sequesters BMPs, making them unavailable for BMP receptor binding. It also binds LRP6, which, while weakly activating the LRP6 pathway, simultaneously blocks Wnt binding with strong Wnt-mediated signaling. Mature human USAG1 is 183 amino acids (aa) in length. It contains one CTCK domain (aa 75-170) plus two N-linked glycosylation sites. Mature human USAG1 (aa 24-206) shares 97% aa identity with mature mouse USAG1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human USAG1 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USAG1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
