| 货号 | AF7004-SP |
| 别名 | fermitin family member 3; KIND3; KIND3fermitin family homolog 3 (Drosophila); Kindlin 3; Kindlin-3; MGC10966; MIG-2; MIG2B; MIG2BURP2SF; MIG2-like protein; UNC-112 related protein 2; UNC112C; Unc-112-related protein 2; URP2; URP2fermitin family homolog 3; URP2SF | 全称 | Fermitin Family Member 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human FERMT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) FERMT2 is observed and less than 1% cross-reactivity with rhFERMT1 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 83706 (Human); 108101 (Mouse); 309186 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FERMT3 Ala498-Phe667 Accession # Q86UX7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | FERMT3 (Fermitin family homolog 3; also kindling-3, MIG2B and URP2) is a 72-78 kDa member of the kindling family of proteins. It is a cytoplasmic protein that is expressed by B cells, T cells, platelets, neutrophils and vascular endothelial cells. FERMT3 acts on the cytoplasmic tails of beta -integrins 1 thru 3 to induce a conformational change and activation. This allows for expressing cell adhesion and arrest. Human FERMT3 is 667 amino acids (aa) in length. It contains a poly-Lys region (aa 147-155) plus a FERM domain (aa 229-558) that itself possesses a membrane-anchoring Pleckstrin homology domain (aa 354-457). There are three utilized phosphorylation sites at Ser8, Tyr11 and Tyr504. There are also two splice variants. One is 56 kDa in size and represents the use of an alternative start site at Met181, while a second contains a deletion of aa 360-363. Over aa 498-667, human FERMT3 shares 99% aa identity with mouse FERMT3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FERMT3 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human FERMT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7004) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FERMT3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
