| 货号 | AF6999-SP |
| 别名 | Hexabrachion-like protein; HXBL; tenascin XB; tenascin XB2; tenascin-X; TNX; TNXB; TNXB1growth-inhibiting protein 45; TNXB2tenascin XB1; TNXBS; TNXBSTNXTENX; XB; XBS; XB-S; XBTN-X |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(50 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human Tenascin X isoform 2 in direct ELISAs and human Tenascin X in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Tenascin R is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 50 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7148 (Human); 81877 (Mouse); 415089 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Tenascin X isoform 2 Met1-Gly673 Accession # AAH33740 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Tenascin-XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Tenascin X by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Tenascin X in Human Lung Mesothelioma. Tenascin X was detected in immersion fixed paraffin-embedded sections of human lung mesothelioma using Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Tenascin X by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Tenascin X at approximately 400 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system. |
