| 货号 | AF6907-SP |
| 别名 | Caldecrin; chymotrypsin C (caldecrin); chymotrypsin-C; CLCR; CLCRserum calcium decreasing factor; CTRC; EC 3.4.21; EC 3.4.21.2; ELA4; elastase 4; Elastase IV |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Chymotrypsin C/CTRC in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11330 (Human); 76701 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Chymotrypsin C/CTRC Cys17-Leu268 Accession # Q99895 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Chymotrypsin C(CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. The CTRC gene encodes a serum calcium-decreasing factor that has chymotrypsin-like protease activity (1). CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. The enzyme is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC is a key regulator of activation and degradation of cationic trypsinogen (2, 3). The inappropriate activation of cationic trypsinogen can contribute to the development of pancreatitis (2, 3). CTRC inhibits osteoclast differentiation by suppression of NFATc1 function by a mechanism independent of its proteolytic activity (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Chymotrypsin C/CTRC by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymotrypsin C/CTRC at approximately 33-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1./P | |
Chymotrypsin C/CTRC in Human Pancreas. Chymotrypsin C/CTRC was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
