| 货号 | AF6864-SP |
| 别名 | Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Pro Caspase-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Caspase-2, rhCaspase-3 (long and short forms), rhCaspase-3, rhCaspase-7, rhCaspase-8, rhCaspase-8 p10, rhCaspase-8 p20 and rhCaspase-9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 843 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Pro Caspase-10 Met1-Pro228 Accession # Q92851 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on procaspases-3, 4, 6, 7, and 9. Human procaspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Pro Caspase‑10 by Western Blot. Western blot shows lysates of KG‑1 human acute myelogenous leukemia cell line, human heart tissue, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pro Caspase‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pro Caspase‑10 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Pro Caspase‑10 in Jurkat Human Cell Line. Pro Caspase‑10 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human Pro Caspase‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6864) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
