| 货号 | AF6856-SP |
| 别名 | Aurora Borealis; BORA; C13orf34; chromosome 13 open reading frame 34; FLJ22624; hsBora; protein aurora borealis | 全称 | Protein Aurora Borealis |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Bora in direct ELISAs. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79866 (Human); 777441 (Mouse); 306102 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Bora Gly2-Ala180 Accession # Q6PGQ7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates Bora dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa. There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87. Over aa 2-180, human Bora shares 84% aa identity with mouse Bora. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Bora in MCF‑7 Human Cell Line. Bora was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Bora Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6856) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
