| 货号 | AF6809-SP |
| 别名 | CDK8 protein kinase; Cell division protein kinase 8; cyclin-dependent kinase 8; EC 2.7.11; EC 2.7.11.22; EC 2.7.11.23; K35; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35 | 全称 | Cyclin-dependent Kinase 8 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human CDK8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CDK2, rhCDK4, and CDK6 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1024 (Human); 264064 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CDK8 Asn380-Tyr464 Accession # P49336 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CDK8 (cyclin-dependent kinase 8; also K35) is a 53-55 kDa member of the CDC2/CDKX subfamily, CMGC Ser/Thr protein kinase family of enzymes. Together with cyclin C, MED12 and MED13, CDK8 forms a "CDK8 module" that contributes to the structure of a four module Mediator complex. CDK8 would appear to have multiple functions. It can block transcription, and the re-initiation of transcription; it can also promote the transcription of thyroid hormone target genes; and it appears to phosphorylate the ICD of Notch, promoting its degradation. Human CDK8 is 464 amino acids (aa) in length. It contains one protein kinase domain (aa 21-335) and two utilized phosphorylation sites at Thr410 and Ser413. There are two potential alternative start sites at Met174 and Met61. Over aa 380-464, human CDK8 shares 98% aa identity with mouse CDK8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CDK8 in Human Colon. CDK8 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human CDK8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6809) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
