| 货号 | AF6716-SP |
| 别名 | Alpha-2,8-sialyltransferase 8A; alpha-N-acetylneuraminide alpha-2,8-sialyltransferase; disialoganglioside (GD3) synthase; Ganglioside GD3 synthase; Ganglioside GT3 synthase; ganglioside-specific alpha-2,8-polysialyltransferase; GD3 synthase); GD3S; sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase, GD3synthase) A; sialyltransferase 8A (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase; Sialyltransferase 8A; Sialytransferase St8Sia I; SIAT8A; SIAT8-A; SIAT8EC 2.4.99.8; ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1; ST8SiaI | 全称 | ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6489 (Human); 20449 (Mouse); 25280 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ST8 alpha -2,8‑Sialyltransferase 8A/ST8SIA1 Tyr49-Ser356 Accession # Q92185 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues and are particularly prevalent on neuronal cells (1). Ganglioside GD3 is involved in cell adhesion and the growth of cultured malignant cells (2). ST8SIA1 is a sialyltransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 (NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) to produce GD3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) and GT3 (NeuNAc alpha 2‑8NeuNAc alpha 2‑8NeuNAc alpha 2‑3Gal beta 1‑4Glc‑Cer) in a successive manner (3); therefore the enzyme has both GD3 and GT3 synthase activity (4). ST8SIA1 is mainly expressed in adult and fetal brain, and its expression is enhanced in melanoma cell lines (3, 4, 5). Like most known glycosyltransferases, ST8SIA1 is predicted as a type II transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of the Golgi apparatus. However, recently GD3 synthase activity was demonstrated at the surface of epithelial and melanoma cells, suggesting glycosphingolipid synthesis may occur at the cell membrane (6). Recombinant ST8SIA1 also showed activity on fetuin from fetal calf serum, when measured using a phosphatase-coupled method (7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ ST8SIA1 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line, SK‑Mel‑28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 at approximately 48‑50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 in Human Breast Cancer Tissue. ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 alpha ‑2,8‑Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
