| 货号 | AF670-SP |
| 别名 | GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor | 全称 | Glial Cell line-derived Neurotrophic Factor Receptor alpha 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human GFR alpha ‑3/GDNF R alpha ‑3 in direct ELISAs and Western blots. In direct ELISA and Western blots, no cross-reactivity with recombinant human GFR alpha -2 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2676 (Human); 14587 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional mapping of receptor tyrosine kinases in myxoid liposarcoma. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GFR alpha ‑3/GDNF R alpha ‑3 Met1-Trp382 Accession # AAC24355 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PEP) and artemin (ART), distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of these neurotrophic factors are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and one of four ligand binding cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins (GFR alpha -1, 2, 3 or 4). These four GFR-alpha proteins share conserved placements of many of their cysteine residues. GFR alpha -1, 2, 3 or 4 have been shown to preferentially bind GDNF, NTN, ART and PSP, respectively. While the GFR alpha -3/Ret complex is highly specific for ART, the GFR alpha -1 /Ret complex is extremely promiscuous and may also be utilized by NTN and ART. Human GFR alpha -3 cDNA encodes a 400 amino acid (aa) residue precursor protein with an N-terminal signal peptide and C-terminal hydrophobic domain. GFR alpha -3 is approximately 34% and 36% identical to GFR alpha -1 and GFR alpha -2, respectively. Human and mouse GFR alpha -3 share approximately 76% amino acid sequence homology. The gene encoding GFR alpha -3 has been localized to human chromosome 5. GFR alpha -3 is expressed at high levels only in the developing and adult sensory and sympathetic ganglia of the peripheral nervous system. In the trigeminal ganglions, the expression of GFR alpha -3 is found in a population of neurons distinct from those expressing GFR alpha -1 or 2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GFR alpha ‑3/GDNF R alpha ‑3 by Western Blot. Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human GFR alpha ‑3/GDNF R alpha ‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GFR alpha ‑3/GDNF R alpha ‑3 at approximately 55 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
