| 货号 | AF669-SP |
| 别名 | follistatin isoform FST317; follistatin; FS; FSActivin-binding protein; FST |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse Follistatin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Follistatin inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. At 6 μg/mL, this antibody will neutralize approximately 60-85% of rhFollistatin 300 bioactivity on K562 cells. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10468 (Human); 14313 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential Expression and Release of Activin A and Follistatin in Chronic Rhinosinusitis with and without Nasal Polyps. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human (rh) Follistatin Gly30-Asp329 Accession # P19883 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high‑affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. The recombinant human FS-300 produced at R&D Systems contains 301 aa residues and represents a molecular form derived from human FS‑315 containing a truncation of 15 residues from the carboxy–terminus. FS-288 binds with high‑affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. The binding affinity of R&D Systems’ FS-300 to heparan sulfate has not been determined. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice. Over aa 30-329, human Follistatin shares 97% aa identity with mouse Follistatin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Follistatin Inhibition of Activin A-induced Hemoglobin Expression and Neutralization by Human Follistatin Antibody. Recombinant Human Follistatin 300 (Catalog #669‑FO) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin 300 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669). At 6 μg/mL, this antibody will neutralize approximately 60‑85% of rhFollistatin 300 bioactivity. | |
Follistatin in Human Breast. Follistatin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse Follistatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF669) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
