| 货号 | AF6699-SP |
| 别名 | C1orf136; disrupted in schizophrenia 1 protein; disrupted in schizophrenia 1; FLJ13381; FLJ21640; FLJ25311; FLJ41105; KIAA0457; SCZD9 | 全称 | Disrupted in Schizophrenia 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DISC1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27185 (Human); 244667 (Mouse); 307940 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. DISC1 causes associative memory and neurodevelopmental defects in fruit flies | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DISC1 Lys101-Arg260 Accession # Q9NRI5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | DISC1 (Disrupted in Schizophrenia 1) is a 100-105 kDa cytoplasmic and mitochondrial protein that belongs to no known molecular family. It is widely expressed, and appears to have multiple interaction partners, among which are NDEL1, alpha -tubulin, TRAF3IP1 and GSK-3 beta. DISC1 is of particular interest in the brain where it appears to play a role in both neuronal proliferation and migration. Regarding proliferation, DISC1 inhibits GSK-3 beta activity, resulting in neural progenitor cell proliferation without differentiation. With respect to migration, DISC1 promotes embryonic subventricular neuron migration while inhibiting widespread adult neuronal migration from the hippocampal subgranular layer. Human DISC1 is 854 amino acids (aa) in length. It contains an N-terminal globular domain (aa 1-346) plus four coiled-coil regions (aa 366‑830). DISC1 is phosphorylated and forms homodimers. There are multiple isoforms. Among them is a 65-70 kDa form that shows an 18 aa substitution for aa 661-854, a 48 kDa form that contains a 20 aa substitution for aa 350-854, a 61-64 kDa form that possesses a seven aa substitution for aa 545-854, and a 90 kDa form that contains a deletion of aa 748-769. Over aa 101-260, human DISC1 shares 44% aa identity with mouse DISC1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human DISC1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Daudi human Burkitts lymphoma cell line, and Ramos human Burkitts lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human DISC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6699) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DISC1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
DISC1 in Human Brain. DISC1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human DISC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6699) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
