| 货号 | AF6674-SP |
| 别名 | augmented in rheumatoid arthritis 17; AURA17; Dardarin; DKFZp434H2111; EC 2.7.11.1; FLJ45829; leucine-rich repeat kinase 2; leucine-rich repeat serine/threonine-protein kinase 2; PARK8; PARK8DARDARIN; Parkinson disease (autosomal dominant) 8; RIPK7; ROCO2 | 全称 | Leucine-rich Repeat Ser/Thr Protein Kinase 2 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LRRK2 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 120892 (Human); 66725 (Mouse); 300160 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LRRK2 Val241-Val500 Accession # Q5S007 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LRRK2 (Leu-rich repeat/LRR-containing protein kinase 2; also Dardarin) is a 260-280 kDa member of the TKL (Tyr kinase-like) Ser/Thr protein kinase family of molecules. It is unusual in that it possesses both GTPase and protein kinase enzyme activity. LRRK2 is expressed in dopamine system neurons, macrophages and B2 B cells (in rodent), and mutations of the molecule are associated with the pathogenesis of Parkinsons disease. Through its protein kinase activity, LRRK2 phosphorylates both 4E-BP and FoxO1, two molecules involved in the initiation of protein translation. Human LRRK2 is 2527 amino acids (aa) in length. It contains multiple domains, including 16 LRRs (aa 226-249; 791-1291; 1556-1887), one coiled-coil region (aa 319-348), a GTPase Roc domain (aa 1328-1511), a protein kinase region (aa 1879-2138), and a WD40 domain (aa 2168-2387). There are three potential splice variants for LRRK2. One shows an alternative start site at Met1203 coupled to a 26 aa substitution for aa 2465-2527, a second contains a three aa substitution for aa 1-1165, and a third possesses an alternative start site at Met402 accompanied by a 12 aa substitution for aa 1259-2527. Over aa 241-500, human LRRK2 shares 85% aa identity with mouse LRRK2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
LRRK2 in Human Brain. LRRK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human LRRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6674) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hemotoxylin (blue). Specific staining was localized to cytoplasm and processes of neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
