Human DNAM-1/CD226 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human DNAM-1/CD226 Affinity Purified Polyclonal Ab (25 UG)

货号： AF666-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF666-SP
别名	CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
全称	DNAX Accessory Molecule 1
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human DNAM-1/CD226 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize DNAM‑1-mediated adhesion of the COLO 205 human colorectal adenocarcinoma cell line. Shibuya, A. et al. (1996) Immunity 4:573. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 20 µg/mL Recombinant Human DNAM-1/CD226 Fc Chimera.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human DNAM-1/CD226 Glu19-Asn247 Accession # Q15762
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359. Shibuya, A. et al. (1996) Immunity 4:573. Bottino, C. et al. (2003) J. Exp. Med. 198:557. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533. Dardalhon, V. et al. (2005) J. Immunol. 175:1558. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491. Morimoto, K. et al. (2007) Oncogene July 16 epub. Shibuya, K. et al. (1999) Immunity 11:615. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

参考图片

Cell Adhesion Mediated by DNAM-1/CD226 and Neutralization by Human DNAM-1/
CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM‑1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM‑1/
CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND₅₀ is typically
0.5‑1.5 µg/mL.