Human PKC zeta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human PKC zeta Affinity Purified Polyclonal Ab (25 UG)

货号： AF6666-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6666-SP
别名	EC 2.7.11; EC 2.7.11.13; nPKC-zeta; PKC2; PKC2PKC-ZETA; PRKCZ; protein kinase C zeta type; protein kinase C, zeta
全称	Protein Kinase C zeta
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human PKC zeta in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5590 (Human); 18762 (Mouse); 25522 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PKC zeta Ile454-Val592 Accession # Q05513
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptor Intracellular Signaling Diabetes Epithelial Cell Polarity Glucose Homeostasis Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neural Stem Cell Markers PDGF Family TNF Superfamily Regulators VEGF Family Wnt Intracellular Signaling

参考图片

Detection of Human PKC zeta by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, MCF‑7 human breast cancer cell line, and human lung tissue. PVDF Membrane was probed with 1 µg/mL of Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.