| 货号 | AF6665-SP |
| 别名 | EC 2.7.13.3; Histidine protein kinase NDKB; MGC2212; NDK B; NDKB; NDP Kinase B2; NDPKB; NDPK-B; NM23B; nm23-H2; NME2; non-metastatic cells 2, protein (NM23B) expressed in; nucleoside diphosphate kinase B; protein (NM23) expressed in | 全称 | Non-metastatic Protein 23 Homolog 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NM23-H2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NM23‑H1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4831 (Human); 18103 (Mouse); 83782 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NM23-H2 Ala2-Glu152 Accession # P22392 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | NM23-H2 or NME-2 (non-metastatic cell 2; also Nucleoside diphosphate kinase B, nm23-H2/B, NDPK-B and PUF) is a 17-19 kDa class 1 member of the NME/NDPK family of molecules. It is widely expressed, found in both cytosol and nucleus, and participates in multiple activities. In conjunction with nm23-H1, NME-2 forms homo- and heterohexamers (three dimers or two trimers) that mediate the transfer of phosphate from ATP to nucleoside diphosphates. It also serves as a transcriptional regulator of genes such as c-myc and PDGF-A. Human NME-2 is 152 amino acids (aa) in length. It contains a kinase region (aa 5-134), two potential phosphorylation sites (Tyr52 and Thr94), a phosphohistidine residue at position 118, and multiple lysine acetylation sites. There is one potential NME-2 splice form that shows a six aa substitution for aa 77-152. The genes for NME-1 and NME-2 are adjacent, and an unusual splicing event generates a 33 kDa fusion protein (NM23-LV) that is composed of aa 1-114 of NME-1 plus a Thr insert that is N-terminally coupled to full-length NME-2. Full-length human NME-2 shares 88% and 98% aa identity with human NME-1 and mouse NME-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human NM23‑H2 by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and MCF‑7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NM23‑H2 Polyclonal Antibody (Catalog # AF6665) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23‑H2 at approximately 20 and 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
NM23‑H2 in MCF‑7 Human Cell Line. NM23‑H2 was detected in immersion fixed MCF‑7 human breast cancer cell line using Sheep Anti-Human NM23‑H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6665) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
