| 货号 | AF6664-SP |
| 别名 | B3GN-T1; beta-1,3-N-acetylglucosaminyltransferase bGnT-6; BETA3GNTI; EC 2.4.1.149; i-beta-1,3-N-acetylglucosaminyltransferase; iGAT; iGNT; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1B3GNT6; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 | 全称 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1/2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse beta -1,3-N-acetylglucosaminyltransferase 1/B3GNT1 in Western blot. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11041 (Human); 108902 (Mouse); 293667 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 1/B3GNT1 Asp43-Cys415 Accession # O43505 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the beta 3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse beta -1,3-N-acetylglucosaminyltransferase 1/B3GNT1 by Western Blot. Western blot shows lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of Human beta -1,3-N-acetylglucosaminyltransferase 1/B3GNT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -1,3-N-acetylglucosaminyltransferase 1/B3GNT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
