| 货号 | AF6659-SP |
| 别名 | calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; lysophospholipase; MGC126350; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4cytosolic phospholipase A2 | 全称 | Phospholipase A2 Group IV A |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human and mouse PLA2G4A in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5321 (Human); 18783 (Mouse); 24653 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf21-derived recombinant human PLA2G4A Met1-Ala749 Accession # NP_077734 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PLA2G4A (Phospholipase A2 Group IVA; also cytosolic phospholipase A2 alpha/cPLA2 alpha and lysophospholipase) is an 100-110 kDa member of the cytosolic PLA2 family of enzymes. Expression can be induced in select cell types, including endothelium, smooth muscle, macrophages, PMNs, fibroblasts, mast cells and platelets. Tumor cells often serve as sources for cPLA2 alpha activity. cPLA2 alpha is synthesized in the cytosol where, upon activation, it translocates to sites containing cell membranes, including the Golgi, ER and nuclear membrane. This translocation allows for enzymatic action on membrane phospholipids, generating free arachidonic acid that is converted into proinflammatory eicosanoids. Human cPLA2 alpha is 749 amino acids (aa) in length. It contains a phospholipid-binding and membrane-association C2 domain (aa 1-178), and an overlapping PLA2c domain that demonstrates catalytic activity (aa 140-740). While phosphorylation on Ser505 and Ser727 contribute to activation, there are at least five other utilized phosphorylation sites on the molecule. Phosphorylation may increase the MW of cPLA2 alpha in SDS-Page to about 100 kDa. Full-length (aa 1-749) human PLA2G4A/cPLA2 alpha shares 94% aa sequence identity with mouse PLA2G4A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse PLA2G4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse PLA2G4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6659) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLA2G4A at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
