| 货号 | AF6648-SP |
| 别名 | cancer/testis antigen 97; CO16; CT97; FLJ35226; HSJ001348; ly-6K; lymphocyte antigen 6 complex, locus K; lymphocyte antigen 6K | 全称 | Lymphocyte Antigen 6 Complex Locus K |
| 反应种属 | Human |
| 应用 | Western Blot(0.25 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Ly6K in Western blots. |
| 使用方法 | Western Blot: 0.25 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54742 (Human); 76486 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ly6E/K Signaling to TGFbeta Promotes Breast Cancer Progression, Immune Escape, and Drug Resistance. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Ly6K Asp18-Gly138 Accession # Q17RY6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Ly6K by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 0.25 µg/mL of Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Ly6K in Human Lung Cancer Tissue. Ly6K was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
