| 货号 | AF6614-SP |
| 别名 | a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 8; ADAM metallopeptidase with thrombospondin type 1 motif, 8; ADAMTS-8; ADAM-TS8A disintegrin and metalloproteinase with thrombospondin motifs 8; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82; FLJ41712; METH-2; METH2ADAM-TS 8; METH-8 | 全称 | A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 8 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human ADAMTS8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ADAMTS1 is observed and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS3, rhADAMTS4, rhADAMTS5, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11095 (Human); 30806 (Mouse); 300475 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS8 Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala) Accession # AAD48081 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | ADAMTS8 (A disintegrin and metalloprotease with thrombospondin motifs 8; also METH-2) is a 95 kDa member of an ADAMTS subfamily of Zn metalloproteases that includes ADAMST-1, -4, -5 and -15. It is expressed by chrondrocytes, neurons, astrocytes and macrophages, and likely participates in proteoglycan (aggrecan) proteolysis. Human proADAMTS8 is a secreted, 863 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 27-213), a peptidase M12B domain (aa 219-429), a disintegrin region (aa 438-525), and two TSP type I sequences (aa 526-888) that are separated by an intervening spacer domain (aa 690-831). Cleavage of the proregion generates a mature 80 kDa molecule that may undergo additional processing to create a 65-67 kDa truncated form. There are two potential splice variants. One shows a 31 aa substitution for aa 411-889, while another shows an alternative start site at Met231. Over aa 29-691, human ADAMTS8 shares 79% aa identity with mouse ADAMTS8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ADAMTS8 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either transfected with human ADAMTS8, mock transfected (-), or transfected with an unrelated vector. PVDF Membrane was probed with 1 µg/mL of Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS8 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
ADAMTS8 in Human Brain. ADAMTS8 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human ADAMTS8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6614) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
