| 货号 | AF6604-SP |
| 别名 | BABAM1; BRISC and BRCA1 A complex member 1; C10orf62; C19orf62; chromosome 19 open reading frame 62; FLJ20571; HSPC142; mediator of Rap80 interactions and targeting 40 kDa; Mediator of RAP80 interactions and targeting subunit of 40 kDa; MERIT40BRCA1-A complex subunit MERIT40; NBA1; NBA1BRISC and BRCA1 A complex member; new component of the BRCA1 A complex; New component of the BRCA1-A complex; new component of the BRCAA1 A complex | 全称 | Mediator of RAP80 Interactions and Targeting Subunit of 40 kDa |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MERIT40 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29086 (Human); 68251 (Mouse); 290631 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MERIT40 Asn182-Val329 Accession # Q9NWV8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | MERIT40 (MEdiator of Rap80 Interactions and Targeting subunit of 40 kDa; also NBA1 and c19orf62 and HSPC142) is a 40 kDa member of the MERIT40 family of proteins. It is ubiquitously expressed, and participates in the DNA damage response. At sites of double strand breaks, histones are phosphorylated, ubiquitinated, and deubiquitinated, with repairs performed by a sequentially formed complex that contains BRCA1, Rap80 and BRE. MERIT40 apparently serves as a scaffold protein that maintains the integrity of the complex. Human MERIT40 is 329 amino acids (aa) in length. It contains one VWF-A domain (aa 95-298) and six utilized Ser/Thr phosphorylation sites. There are three potential splice variants. One shows a 13 aa substitution for aa 234-329, a second possesses a 105 aa substitution for aa 263‑329, while a third shows a 37 aa substitution for aa 1-115, coupled to a deletion of aa 263-329. Over aa 182-329, human MERIT40 shares 95% aa identity with mouse MERIT40. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MERIT40 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gel was loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Human MERIT40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MERIT40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
MERIT40 in Human Breast Cancer Tissue. MERIT40 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human MERIT40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6604) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
