| 货号 | AF6587-SP |
| 别名 | EC 3.4.22; EC 3.4.22.-; Sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2 | 全称 | SUMO-Specific Peptidase 1 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SUMO1-Specific Peptidase 1/SENP1 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29843 (Human); 223870 (Mouse); 300193 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SUMO1-Specific Peptidase 1/SENP1 Glu419-Leu644 Accession # Q9P0U3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SENP1 (Sentrin/SUMO-specific protease) is a member of the SENP family of proteases. Sentrin/SUMO-specific proteases (SENPs) are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length SUMOs to their mature forms and deconjugation of SUMOs from SUMOlyated proteins. The seven mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity. Human SENP-1 has broad specificity for the three mammalian SUMOs. It is found in the cytoplasm and nucleus depending on cell type, and is expressed in testis, thymus, pancreas, spleen, liver, ovary and small intestine. It is thought that localization of SENP-1 is vital for the regulation for SUMOylation status of target proteins. The small ubiquitin-like modifier (SUMO) is a member of ubiquitin-like protein family. SUMO modification of the target proteins is a reversible process that regulates many cellular processes including transcription regulation, nuclear localization, centromere segregation and signal transduction. The recombinant human SENP-1 (immunogen) includes the catalytic domain, which has been shown to be sufficient for SENP-1 activity and substrate specificity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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SUMO1-Specific Peptidase 1/SENP1 in Human Testis. SUMO1-Specific Peptidase 1/SENP1 was detected in immersion fixed paraffin-embedded sections of human testis using Sheep Anti-Human SUMO1-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to late spermatids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
