| 货号 | AF6558-SP |
| 别名 | Cancer/testis antigen 80; CT80; CT80mili; FLJ10351; HILI; HILIMiwi like; MGC133049; MILI; PIWIL1L; piwil2-like protein; piwi-like 2 (Drosophila); piwi-like protein 2 | 全称 | Piwi-like Protein 2 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PIWIL2 in direct ELISAs and human and rat PIWIL2 in Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55124 (Human); 57746 (Mouse); 306011 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential L1 regulation in pluripotent stem cells of humans and apes. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PIWIL2 Lys216-Asp379 Accession # Q8TC59 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44+CD24- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat PIWIL2 by Western Blot. Western blot shows lysates of rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human PIWIL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
