| 货号 | AF6557-SP |
| 别名 | diphosphate phosphohydrolase; EC 3.6.1.1; inorganic diphosphatase; inorganic pyrophosphatase 1; inorganic pyrophosphatase; IOPPP; IOPPPMGC111556; IPP1; PP1; PPase; PPcytosolic inorganic pyrophosphatase; pyrophosphatase (inorganic) 1; pyrophosphatase (inorganic); pyrophosphatase 1; Pyrophosphate phospho-hydrolase; SID6-8061 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat Inorganic Pyrophosphatase/PPA1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5464 (Human); 852296 (Yeast) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Inorganic Pyrophosphatase/PPA1 Met1-Asn289 Accession # Q15181 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PPA1 (Inorganic pyrophosphatase 1; also PPase and IOPPP) is a 32-36 kDa cytoplasmic member of the PPase family of enzymes. It is ubiquitously expressed, and acts on di- (or pyro) phosphate, generating orthophosphate in a Mg+-dependent manner. This activity can both generate energy for cells, or in the case of osteoblasts, provide raw material for calcification. The consumption of pyrophosphate may also remove inhibitors of enzymes such as guanylyl cyclase, and PPA1 itself is also reported to stimulate gene expression. Human PPA1 is 289 amino acids (aa) in length. There is one pyrophosphatase domain between aa 42-255, and two utilized acetylation sites at Lys 57 and Lys228. PPA1 is known to form homodimers. There is one potential alternative start site at Met46. Full-length human PPA1 shares 94% aa sequence identity with mouse PPA1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Inorganic Pyrophosphatase/PPA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, IMR‑32 human neuroblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Inorganic Pyrophosphatase/PPA1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
