| 货号 | AF6548-SP |
| 别名 | HIWI; HIWIpiwi homolog; MIWI; piwi (Drosophila)-like 1; PIWI; piwi-like 1 (Drosophila); piwi-like protein 1 | 全称 | Piwi-like Protein 1 |
| 反应种属 | Human/Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PIWIL1/HIWI in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9271 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PIWIL1/HIWI Arg82-Thr290 Accession # Q96J94 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
PIWIL1/HIWI in Mouse Testes. PIWIL1/HIWI was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Human PIWIL1/HIWI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6548) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
