| 货号 | AF6525-SP |
| 别名 | IGLC; IGLC2; immunoglobulin lambda constant 2 (Kern-Oz- marker); MGC20392; MGC45681 | 全称 | Immunoglobulin lambda C Domain |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects recombinant human IGLC2 in direct ELISAs and Western blots. Detects endogenous Immunoglobulin lambda constant region in natural samples by Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3538 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IGLC2 Gln2-Ser106 Accession # P0CG05 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Immunoglobulin lambda constant region is a Ke-Oz- component of immunoglobulin (Ig) lambda light chains and is found C-terminal to the joining region of the light chain. IGLC2 is 105 amino acids (aa) in length. It contains one Ig-like domain (aa 7-100) and generates a disulfide bond with an IgH chain via Cys104. One potential variant shows a three aa substitution for aa 7-9. The closest mouse lambda ortholog shares 61% aa identity with full-length human IGLC2. IGLC2 shares 93 - 98 % aa identity with IGLC1, IGLC3 and IGLC7. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human Immunoglobulin Lambda Constant Region by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
